FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Sxl, mei-P26
FlyBase ID
FBig0000101910
Interaction Network
Interactions Browser links
Sxl network
mei-P26 network
Reported Interactions
FBrf0221101-5.coIP.WB
Reference
Li et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

Mei-P26

bait
Sxl 

Sxl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2013)
FBrf0221101-9.CH.WB
Reference
Li et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

Mei-P26

neutral component
mei-P26 

Mei-P26

neutral component
mei-P26 

Mei-P26

neutral component
Sxl 

Sxl

neutral component
Sxl 

Sxl

neutral component
Sxl 

Sxl

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mei-P26, GFP-bgcn, Sxl and HA-bam cofractionate over a range of Superdex G-200 gel filtration fractions.

(Li et al., 2013)

Source was adult female ovaries of transgenic fly line; proteins produced from endogenous genes.

(Li et al., 2013)
FBrf0237766-12.coIP.WB
Reference
Malik et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait

FLAG tag

mei-P26 

Mei-P26

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Malik et al., 2017)
FBrf0237766-7.Y2H
Reference
Malik et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

Mei-P26

bait

fused to lexA DNA-binding domain

Sxl 

Sxl

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Malik et al., 2017)

Two-hybrid system: yeast lexA-BD/GAL4-AD

(Malik et al., 2017)
FBrf0237766-9.Y2H
Reference
Malik et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait

fused to lexA DNA-binding domain

mei-P26 

Mei-P26

prey

fused to GAL4 activation domain

mei-P26 
mei-P26 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-P26 
NHL domain
sufficient binding region
aa 921-1140
mei-P26 
NHL domain
necessary binding region
aa 921-1140
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Malik et al., 2017)

Two-hybrid system: yeast lexA-BD/GAL4-AD

(Malik et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)