FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nelf-E, Nelf-A
FlyBase ID
FBig0000101985
Interaction Network
Interactions Browser links
Nelf-E network
Nelf-A network
Reported Interactions
FBrf0227292-428.TFIN
Reference
Rhee et al., 2014
Description
physical association
Assay
Collection
TF_Interaction_Network-2
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Nelf-E 

FBgn0017430

unspecified role
Nelf-E 

FBgn0017430

unspecified role
Nelf-E 

FBgn0017430

unspecified role
Nelf-A 

FBgn0038872

unspecified role
Nelf-A 

FBgn0038872

unspecified role
Nelf-A 

FBgn0038872

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

(Rhee et al., 2014)

HGScore = 18.539197

(Rhee et al., 2014)
FBrf0247767-2.coIP.WB
Reference
Mazina et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nelf-A 

NELF A

bait
Nelf-E 

NELF E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mazina et al., 2021)
FBrf0247767-7.coIP.WB
Reference
Mazina et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nelf-E 

NELF E

bait
Nelf-A 

NELF A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mazina et al., 2021)
FBrf0257712-12.PD.MW
Reference
Dollinger et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nelf-E 

NelfE

bait

His tag

Nelf-A 

NelfA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as a recombinant fusion protein in a baculovirus and insect cell system.

(Dollinger et al., 2023)

The NELF complex was tandem purified with Nelf-E affinity purification followed by immunoprecipitation with TH1-anti-FLAG antibody. Nelf-A, NELF-B, TH1, and Nelf-E captured as 3 pairwise interactions.

(Dollinger et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)