FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
AGO1, smg
FlyBase ID
FBig0000102017
Interaction Network
Interactions Browser links
AGO1 network
smg network
Reported Interactions
FBrf0220359-1.coIP.WB
Reference
Pinder and Smibert, 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 4embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 3embryonic cycle 4embryonic cycle 9embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

Smaug

bait
AGO1 

Ago1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Pinder and Smibert, 2013)

Immunoprecipitated in the presence of RNaseA.

(Pinder and Smibert, 2013)
FBrf0220359-4.coIP.WB
Reference
Pinder and Smibert, 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 4embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 3embryonic cycle 4embryonic cycle 9embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

smg 

Smaug

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Pinder and Smibert, 2013)

The interaction of AGO1 with smg is not disrupted by mutations in AGO1 (K657A, K694A) that disrupt its binding to miRNAs.

(Pinder and Smibert, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)