FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Chi, ap
FlyBase ID
FBig0000102035
Interaction Network
Interactions Browser links
Chi network
ap network
Reported Interactions
FBrf0098853-1.Y2H
Reference
Morcillo et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chi 

Chip

bait

fused to LexA DNA-binding domain

ap 

Ap

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Morcillo et al., 1997)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Morcillo et al., 1997)
FBrf0125233-1.PD.A
Reference
Torigoi et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chi 

Chip

bait

GST tag

Chi 
ap 

Apterous

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Chi 
LIM interaction domain
necessary binding region
aa 472-577
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Torigoi et al., 2000)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)