Source was cell extract of baculovirus-infected Sf21 cell line; bait produced from transfected construct; prey produced from transfected construct.

Kdm4A coimmunoprecipitates with Su(var)205 (HP1a), but not with HP1b or HP1c. This interaction requires an intact Su(var)205 chromoshadow domain and the HP1a-binding PxVxL motif.

Su(var)205 stimulates Kdm4A H3K36me3 demethylation activity in vitro. In vivo, both Kdm4A and Su(var)205 mutants display increased H3K36me3 levels.

Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Purified peptides were eluted by on-bead trypsin digestion and mass spectrometric analysis.

Enrichment of prey protein was compared to input, mock and unrelated BioTAP affinity purifications using a statistical method (Bamse) developed by the authors to control for multiple sources of bias.