FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cenp-C, Spc105R
FlyBase ID
FBig0000102199
Interaction Network
Interactions Browser links
Cenp-C network
Spc105R network
Reported Interactions
FBrf0213151-1.coIP.MS
Reference
Przewloka et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENPC

bait

ProtA tag

Cenp-C 
Spc105R 

Spc105

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
N-terminal region
sufficient binding region
aa 1-788
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Przewloka et al., 2011)
FBrf0227269-24.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

Protein A tag

Spc105R 

Spc105/KNL1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0231098-27.CH.MW
Reference
Liu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 
Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 
Spc105R 

Spc105R

neutral component
Spc105R 
Spc105R 

Spc105R

neutral component
Spc105R 

Spc105R

neutral component
Spc105R 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
N-terminal region
sufficient binding region
aa 1-105
Spc105R 
C-terminal region
sufficient binding region
aa 1707-1960
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified Cenp-C was mixed with either the Spc105R-Kmn1-Mis12-Nnf1a or Spc105R-Kmn1-Mis12-Nnf1b tetramer, and interaction was assessed by gel filtration and analytical centrifugation.

(Liu et al., 2016)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Liu et al., 2016)

Cenp-C can bind either the Spc105R-Kmn1-Mis12-Nnf1a or Spc105R-Kmn1-Mis12-Nnf1b tetramer to form a complex that is 106.5 kDa or 109.3 kDa in size, respectively.

(Liu et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)