FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cenp-C, Kmn1
FlyBase ID
FBig0000102236
Interaction Network
Interactions Browser links
Cenp-C network
Kmn1 network
Reported Interactions
FBrf0213151-9.coIP.MS
Reference
Przewloka et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENPC

bait

ProtA tag

Cenp-C 
Kmn1 

Nsl1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
N-terminal region
sufficient binding region
aa 1-788
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Przewloka et al., 2011)
FBrf0227269-28.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

Protein A tag

Kmn1 

Nsl1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0231098-16.CH.MW
Reference
Liu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 
Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 
Kmn1 

Kmn1

neutral component

Hpc tag

Kmn1 

Kmn1

neutral component

Hpc tag

Kmn1 

Kmn1

neutral component

Hpc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
N-terminal region
sufficient binding region
aa 1-105
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified Cenp-C was mixed with either the Kmn1-Mis12-Nnf1a or Kmn1-Mis12-Nnf1b trimer, and interaction was assessed by gel filtration and analytical centrifugation.

(Liu et al., 2016)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Liu et al., 2016)

Cenp-C can bind either the Kmn1-Mis12-Nnf1a or Kmn1-Mis12-Nnf1b trimer to form a complex that is 76.4 kDa or 75.6 kDa in size, respectively.

(Liu et al., 2016)
FBrf0231098-20.XL.MS
Reference
Liu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 
Cenp-C 

CENP-C

neutral component

MBP tag

Cenp-C 
Kmn1 

Kmn1

neutral component

Hpc tag

Kmn1 

Kmn1

neutral component

Hpc tag

Kmn1 

Kmn1

neutral component

Hpc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
N-terminal region
sufficient binding region
aa 1-105
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified Cenp-C was mixed with either the Kmn1-Mis12-Nnf1a or Kmn1-Mis12-Nnf1b trimer, then purified by gel filtration. The complex was then treated with BS2G bi-functional crosslinking reagent, and cross-links were mapped by mass spectrometry.

(Liu et al., 2016)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Liu et al., 2016)
FBrf0231099-24.coIP.MS
Reference
Richter et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

GFP tag

Cenp-C 
Cenp-C 
Kmn1 

Nsl1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
N-terminal region
sufficient binding region
aa 1-94
Cenp-C 
N-terminal region residues 26-29
mutation disrupting interaction
aa 26-29

F26A, F29A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Richter et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)