FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
flfl, Pp4-19C
FlyBase ID
FBig0000102237
Interaction Network
Interactions Browser links
flfl network
Pp4-19C network
Reported Interactions
FBrf0227269-16.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flfl 

Falafel

bait

Protein A tag

Pp4-19C 

PP4c

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0227269-3.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
flfl 

Falafel

bait

Protein A tag

Pp4-19C 

PP4c

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0227269-43.coIP.WB
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Pp4-19C 

PP4

bait

FLAG tag

flfl 

Falafel

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A phosphatase-dead version of Pp4-19C (D85N and H115N) binds flfl and PPP4R2r as well as wild-type.

(Lipinszki et al., 2015)

RNAi depletion of Pp4-19C reduces the level of non-phosphorylated flfl in S2 cells.

(Lipinszki et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)