FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
MED19, Ubx
FlyBase ID
FBig0000102268
Interaction Network
Interactions Browser links
MED19 network
Ubx network
Reported Interactions
FBrf0224861-14.PD.A
Reference
Boube et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MED19 

Med19

bait

GST tag

Ubx 

Ubx

prey

[35]S label

Ubx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
homeodomain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Boube et al., 2014)

MED19 can discriminate between different homeodomains. GST-MED19 pulled down Antp, Dfd, Ubx and Abd-B homeodomain peptides, but not en or hth peptides.

(Boube et al., 2014)
FBrf0224861-16.FC.FM
Reference
Boube et al., 2014
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

Venus tag, N-terminal fragment

MED19 

Med19

prey

Venus tag, C-terminal fragment

MED19 
MED19 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
MED19 
homeodomain-interacting motif (HIM)
sufficient binding region
aa 155-229

\'HIM\'

MED19 
homeodomain-interacting motif (HIM)
necessary binding region
aa 160-229

\'ΔHIM\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Boube et al., 2014)
FBrf0224861-17.coIP.WB
Reference
Boube et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
MED19 

Med19

bait

Venus tag, C-terminal fragment

MED19 
Ubx 

Ubx

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
MED19 
homeodomain-interacting motif (HIM)
necessary binding region
aa 160-229

\'ΔHIM\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Boube et al., 2014)
FBrf0224861-4.PD.A
Reference
Boube et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

GST tag

MED19 

Med19

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Boube et al., 2014)
FBrf0224861-9.FC.FM
Reference
Boube et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

Venus tag, N-terminal fragment

Ubx 
MED19 

Med19

prey

Venus tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
homeodomain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fixed embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Boube et al., 2014)
FBrf0245137-2.PL.WB
Reference
Carnesecchi et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

Myc, BirA tag

MED19 

Med19

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Carnesecchi et al., 2020)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Carnesecchi et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)