FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sau, Rab11
FlyBase ID
FBig0000102292
Interaction Network
Interactions Browser links
sau network
Rab11 network
Reported Interactions
FBrf0224860-6.coIP.WB
Reference
Sechi et al., 2014
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab11 

Rab11

bait

GFP tag

sau 

GOLPH3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult male testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sechi et al., 2014)
FBrf0224860-8.PD.WB
Reference
Sechi et al., 2014
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sau 

GOLPH3

bait

GST tag

Rab11 

Rab11

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult testis extract.

(Sechi et al., 2014)
FBrf0224860-9.SPR
Reference
Sechi et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sau 

GOLPH3

bait
Rab11 

Rab11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sechi et al., 2014)

Both proteins produced as GST-fusions, with GST domain cleaved off before analysis.

(Sechi et al., 2014)
FBrf0234570-3.PD.WB
Reference
Sechi et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab11 

Rab11

bait

GST tag

sau 

GOLPH3

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sechi et al., 2017)

sau binds more strongly to Rab11 in the presence of GTP than GDP.

(Sechi et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)