Caf1-180 interacts with Su(var)205 but not with Su(var)3-9 in this study.
Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Purified peptides were eluted by on-bead trypsin digestion and mass spectrometric analysis.
Enrichment of prey protein was compared to input, mock and unrelated BioTAP affinity purifications using a statistical method (Bamse) developed by the authors to control for multiple sources of bias.
Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
coordinates relative to Su(var)205-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 nuclear extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; produced as a recombinant fusion protein in bacterial system.
