FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
spag, Hsp23
FlyBase ID
FBig0000102333
Interaction Network
Interactions Browser links
spag network
Hsp23 network
Reported Interactions
FBrf0224244-8.coIP.MS
Reference
Benbahouche et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
spag 

Spag

bait

FLAG tag

spag 
Hsp23 

CG4463

prey

10 peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spag 
TPR motifs
necessary binding region
aa 96-197
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was soluble cytosolic extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Benbahouche et al., 2014)

Affinity purification of bait and identification of prey by MALDI peptide mapping.

(Benbahouche et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)