FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
spag, Pih1D1
FlyBase ID
FBig0000102408
Interaction Network
Interactions Browser links
spag network
Pih1D1 network
Reported Interactions
FBrf0224244-16.coIP.WB
Reference
Benbahouche et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Pih1D1 

dPih1D1

bait

FLAG tag

spag 

Spag

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Benbahouche et al., 2014)
FBrf0224244-3.Y2H
Reference
Benbahouche et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stageovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spag 

spag

bait

fused to GAL4 DNA-binding domain

Pih1D1 

CG5792

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo or ovary cDNA expression library).

(Benbahouche et al., 2014)

The spag TPR motifs (aa 96-197) is dispensable for the spag-Pih1D1 interaction.

(Benbahouche et al., 2014)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Benbahouche et al., 2014)
FBrf0239380-7.coIP.WB
Reference
Zur Lage et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
spag 

Rpap3

bait

FLAG tag

Pih1D1 

Pih1d1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zur Lage et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)