Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The BLRP tag is biotinylated in vivo by cotransfection of the BirA enzyme. Biotinylated protein is purified by streptavidin pull down.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

cid, cal1 and His4 interact together as part of a complex.

Proteins were coexpressed in bacterial cells, affinity purified by virtue of the His tag and further purified by gel filtration.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

cid and His4 form a complex with each other, which can additionally interact with cal1.

Proteins were separately purified, mixed and refolded. Complex formation was examined using gel filtration.

Trimeric complex consisting of cal1, cid and His4 was captured as three binary interactions.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Trimeric complex consisting of cal1, cid and His4 was captured as three binary interactions.

Trimeric complex consisting of cal1, cid and His4 was captured as three binary interactions.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.