Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
TAP tandem affinity purification of bait and identification of prey by mass spectrometry.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.