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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102572
Interaction Network
Interactions Browser links
osk network
vas network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0089980-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

Osk

bait

fused to LexA DNA-binding domain

osk 
osk 
vas 

Vas

prey

fused to B42 activation domain

vas 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
region
unspecified role
aa 290-606aa 290-450

necessary: deletion abolishes interaction

vas 
N-terminal region
unspecified role
aa 1-320
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA/B42.

The N-terminus of the long osk protein isoform inhibits the interaction with vas protein.

Binding of osk protein is stronger with vas N-terminal region than with full length vas protein.

FBrf0089980-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

GST-Osk292

bait

GST tag

osk 
vas 

Vas

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
region
unspecified role
aa 292-606
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down from ovary extract.

Bait expressed and purified from bacterial cells.

Prey endogenous to ovary extract.

FBrf0089980-5.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

GST-Osk292

bait

GST tag

osk 
vas 

Vas

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
region
unspecified role
aa 292-606aa 457aa 561aa 593

necessary: the osk6B10 S457F mutation abolishes interaction

necessary: the osk3 N561Y mutation reduces interaction

necessary: the osk5 R593Q mutation abolishes interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacterial cells.

Prey derived from in vitro translation.

FBrf0125216-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

VAS

bait

fused to LexA DNA-binding domain

osk 

Oskar

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

8 of 145 clones recovered in the screen for vas interactors represented osk.

Two-hybrid system: yeast LexA-BD/B42-AD

Positive control.

FBrf0206294-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

Oskar

bait

fused to LexA DNA-binding domain

vas 

Vasa

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovarian cDNA expression library).

119 of 480 positive clones recovered in the osk two hybrid screen corresponded to vas.

Two-hybrid system: yeast LexA-BD/B42-AD

FBrf0212588-2.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GST tag

vas 
osk 

Osk

prey

S tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vas 
Osk-binding region
sufficient binding region
aa 163-319

coordinates relative to vas-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

FBrf0229153-6.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

Osk

bait

GST tag

osk 
vas 

Vasa

prey

His tag

vas 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
LOTUS domain
sufficient binding region
aa 139-240

coordinates relative to osk-PA

vas 
helicase core
sufficient binding region
aa 200-623

coordinates relative to vas-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0229153-7.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

Osk

bait
osk 
vas 

Vasa

prey
vas 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
LOTUS domain
sufficient binding region
aa 139-240

coordinates relative to osk-PA

vas 
helicase core
sufficient binding region
aa 200-623

coordinates relative to vas-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0229153-8.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

Osk

bait

fused to C-terminal ubiquitin fragment and LexA

osk 
vas 

Vasa

prey

fused to N-terminal ubiquitin fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
LOTUS domain
sufficient binding region
aa 139-240

coordinates relative to osk-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast split-ubiquitin system

FBrf0229754-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

fused to GAL4 DNA-binding domain

osk 

Osk

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD via strain Y187 using a lacZ UAS reporter.

FBrf0229754-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

fused to GAL4 DNA-binding domain

vas 
osk 

Osk

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vas 
helicase superfamily C-terminal domain residues 460-621
sufficient binding region
aa 460-621
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD via strain Y187 using a lacZ UAS reporter.

FBrf0229754-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GFP tag

osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0229754-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GFP tag

vas 
osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vas 
helicase superfamily C-terminal domain residues 460-621
sufficient binding region
aa 460-621
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0229754-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GFP tag

vas 
osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vas 
helicase superfamily C-terminal domain residues 460-661
sufficient binding region
aa 460-661

Includes HELIC domain plus 40 additional residues at the C-terminal end.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0233939-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

vas

bait

fused to GAL4 DNA-binding domain

osk 

Oskar

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0234117-1.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

Osk

bait

FLAG tag

osk 
vas 

VASA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0235682-1.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait

GST tag

osk 
osk 
osk 
vas 

vas

prey
vas 
vas 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
LOTUS domain
sufficient binding region
aa 139-240

coordinates relative to osk-PA

osk 
LOTUS domain
necessary binding region
aa 223-240

coordinates relative to osk-PA

osk 
LOTUS domain
mutation disrupting interaction
aa 162aa 228

A162E, coordinates relative to osk-PA

L228E, coordinates relative to osk-PA

vas 
interaction domain
sufficient binding region
aa 200-661
vas 
interaction domain
mutation disrupting interaction
aa 504aa 508

F504E

F508E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0235682-4.Y2H
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait

fused to NubG

osk 
vas 

vas

prey

fused to Cub, lexA-VP16

vas 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
interaction domain
sufficient binding region
aa 139-606

coordinates relative to osk-PA

vas 
C-terminal domain
sufficient binding region
aa 461-661
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Membrane yeast two-hybrid system.

FBrf0235682-5.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

unspecified role
osk 

osk

unspecified role
osk 
osk 

osk

unspecified role
osk 
vas 

vas

unspecified role
vas 
vas 

vas

unspecified role
vas 

vas

unspecified role
vas 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
LOTUS domain
sufficient binding region
aa 144-240

coordinates relative to osk-PA

vas 
C-terminal domain
sufficient binding region
aa 463-661
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0235682-6.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

neutral component
osk 

osk

neutral component
osk 
osk 

osk

neutral component
osk 
vas 

vas

neutral component
vas 
vas 

vas

neutral component
vas 

vas

neutral component
vas 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
LOTUS domain
sufficient binding region
aa 139-240

coordinates relative to osk-PA

vas 
interaction domain
sufficient binding region
aa 463-623
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as recombinant fusion proteins in bacterial system.

FBrf0246626-29.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GFP tag

osk 

Osk

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0246626-63.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GFP tag

osk 

Osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (10)