FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
slmb, Cul1
FlyBase ID
FBig0000102621
Interaction Network
Interactions Browser links
slmb network
Cul1 network
Reported Interactions
FBrf0202079-3.coIP.WB
Reference
Smelkinson et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
slmb 

slmb

bait

FLAG tag

slmb 
Cul1 

Cul1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
slmb 
F-box domain
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Smelkinson et al., 2007)
FBrf0221943-20.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slimb

bait

FLAG tag

Cul1 

Cul1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)

Positive control.

(Zhang et al., 2013)
FBrf0222810-1.coIP.WB
Reference
Wong et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slimb

bait

Myc tag

Cul1 

Cul-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Both unmodified and Neddylated lin19 (Cul-1) coimmunoprecipitate with slmb.

(Wong et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Wong et al., 2013)
FBrf0222810-4.coIP.WB
Reference
Wong et al., 2013
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slimb

bait

Myc tag

Cul1 

Cul-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brain of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Wong et al., 2013)
FBrf0247580-4.coIP.MS
Reference
Barbosa et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
slmb 

Slmb

bait

GFP tag

Cul1 

Cul1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Barbosa et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)