FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ubc10, ari-1
FlyBase ID
FBig0000102735
Interaction Network
Interactions Browser links
Ubc10 network
ari-1 network
Reported Interactions
FBrf0128369-1.Y2H
Reference
Aguilera et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubc10 

ubcD10

bait

fused to GAL4 DNA-binding domain

ari-1 

ari-1

prey

fused to GAL4 activation domain

ari-1 
ari-1 
ari-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ari-1 
C terminal region
necessary binding region
aa 130-503

Q129stop

ari-1 
RING finger 1 motif
mutation decreasing interaction
aa 150

C150Y

ari-1 
RING finger 1 motif
sufficient binding region
aa 129-209
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

(Aguilera et al., 2000)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Aguilera et al., 2000)
FBrf0238699-6.coIP.WB
Reference
Tan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ubc10 

UbcD10

bait

GFP tag

ari-1 

Ari-1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tan et al., 2018)
FBrf0238699-7.coIP.WB
Reference
Tan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ari-1 

Ari-1

bait

V5 tag

ari-1 
ari-1 
Ubc10 

UbcD10

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ari-1 
Ring1 domain residue 136
mutation decreasing interaction
aa 136

C136Y

ari-1 
IBR and Ring2 domains residues 187,332
mutation decreasing interaction
aa 187,332

V187E,S332P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tan et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)