FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102774
Interaction Network
Interactions Browser links
Ten-a network
Ten-m network
Reported Interactions
FBrf0218053-1.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ten-m

bait

HA tag

Ten-a

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

Interaction of neuronally expressed Ten-a and muscle expressed Ten-m indicate that the two proteins interact across the neuromuscular junction (NMJ).

Authors co-expressed Myc tagged Ten-a in nerves using the Q system and HA tagged Ten-m in muscles using GAL4. Synaptosome fractions were prepared from body wall fillets.

FBrf0218059-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ten-a

bait

FLAG tag

Ten-m

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Ten-m-Ten-a heterophilic interactions are similar in strength to Ten-a homophilic interactions but much weaker than Ten-m homophilic interactions.

FBrf0218059-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ten-m

bait

FLAG tag

Ten-a

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Ten-m-Ten-a heterophilic interactions are similar in strength to Ten-a homophilic interactions but much weaker than Ten-m homophilic interactions.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)