FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cp190, ZIPIC
FlyBase ID
FBig0000102811
Interaction Network
Interactions Browser links
Cp190 network
ZIPIC network
Reported Interactions
FBrf0227255-10.coIP.MS
Reference
Maksimenko et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait

FLAG tag

ZIPIC 

ZIPIC

prey

8 unique peptides

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ZIPIC enriched 4-fold in Cp190 purification compared to negative control.

(Maksimenko et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Maksimenko et al., 2015)
FBrf0227255-12.coIP.WB
Reference
Maksimenko et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait
ZIPIC 

ZIPIC

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Maksimenko et al., 2015)
FBrf0227255-14.coIP.WB
Reference
Maksimenko et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait
ZIPIC 

ZIPIC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Maksimenko et al., 2015)
FBrf0227255-16.coIP.WB
Reference
Maksimenko et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ZIPIC 

ZIPIC

bait
Cp190 

CP190

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Maksimenko et al., 2015)
FBrf0227255-21.Y2H
Reference
Maksimenko et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait

fused to GAL4 DNA-binding domain

Cp190 
ZIPIC 

ZIPIC

prey

fused to GAL4 activation domain

ZIPIC 
ZIPIC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cp190 
M domain
sufficient binding region
aa 245-468
ZIPIC 
N-terminal region
sufficient binding region
aa 3-167
ZIPIC 
central region
sufficient binding region
aa 162-274
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Maksimenko et al., 2015)

Positive control.

(Maksimenko et al., 2015)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Maksimenko et al., 2015)

Cp190 binds at least two distinct sites in N-terminal region of ZIPIC. The ZIPIC ZAD domain is not sufficient or even necessary for binding to Cp190.

(Maksimenko et al., 2015)
FBrf0227255-23.PD.MW
Reference
Maksimenko et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ZIPIC 

ZIPIC

bait

MBP tag

Cp190 

CP190

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Maksimenko et al., 2015)
FBrf0227255-25.PD.WB
Reference
Maksimenko et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait

His tag

Cp190 
ZIPIC 

ZIPIC

prey

GST tag

ZIPIC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cp190 
M domain and Zinc fingers
sufficient binding region
aa 245-599
ZIPIC 
N-terminal region
sufficient binding region
aa 1-169
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Maksimenko et al., 2015)
FBrf0253430-50.PD.MS
Reference
Kaushal et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

Cp190

bait

GFP tag

ZIPIC 

ZIPIC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.

(Kaushal et al., 2022)
FBrf0258035-3.Y2H
Reference
Golovnin et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

Cp190

bait

fused to GAL4 DNA-binding domain

Cp190 
ZIPIC 

ZIPIC

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cp190 
interaction domain
sufficient binding region
aa 1-245aa 309-390

within the context of aa 1-293; coordinates relative to Cp190-PA

within the context of aa 1-293; coordinates relative to Cp190-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Golovnin et al., 2023)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2023)
FBrf0259958-3.Y2H
Reference
Sokolov et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait

fused to GAL4 DNA-binding domain

Cp190 
ZIPIC 

ZIPIC

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cp190 
interaction domain
sufficient binding region
aa 1-245aa 309-390

coordinates relative to Cp190-PA

coordinates relative to Cp190-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Sokolov et al., 2024)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Sokolov et al., 2024)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sokolov et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)