FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
tut, mei-P26
FlyBase ID
FBig0000103019
Interaction Network
Interactions Browser links
tut network
mei-P26 network
Reported Interactions
FBrf0226802-1.coIP.PCR
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tut 

Tut

bait

TAP tag

mei-P26 

mei-P26

prey

FLAG tag

mei-P26 
mei-P26 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-P26 
3-prime UTR
sufficient binding region
nt 1-844

weaker binding to 549 nt of UTR

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)
FBrf0226802-2.Y3H
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tut 

Tut

bait

fused to activation domain

tut 
mei-P26 

mei-P26

prey

fused to MS2 RNA-stem loop

mei-P26 
mei-P26 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-P26 
3-prime UTR
sufficient binding region
nt 1-844

weaker binding to 549 nt of UTR

tut 
RRM domain
necessary binding region
aa 37-128
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three-hybrid system: yeast LexA-BD/MS2-stem-loop/activation domain

(Chen et al., 2014)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Chen et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)