FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Diap1, eIF4E1
FlyBase ID
FBig0000103064
Interaction Network
Interactions Browser links
Diap1 network
eIF4E1 network
Reported Interactions
FBrf0202860-1.coIP.MS
Reference
Lee et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Diap1 

Diap1

bait

Myc tag, His tag

eIF4E1 

eIF4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lee et al., 2007)
FBrf0202860-2.coIP.WB
Reference
Lee et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

deIF4E

bait
Diap1 

Diap1

prey

GFP tag

Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
eIF4E-binding motif
mutation decreasing interaction
aa 404

L404R

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Lee et al., 2007)
FBrf0202860-3.PD.A
Reference
Lee et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Diap1 

Diap1

bait

GST tag

Diap1 
Diap1 
eIF4E1 

deIF4E

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
central and C-terminal regions
sufficient binding region
aa 111-438
Diap1 
central region
necessary binding region
aa 111-292
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lee et al., 2007)
FBrf0202860-4.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Diap1 

DIAP1

enzyme
Diap1 

DIAP1

enzyme
eIF4E1 

deIF4E

enzyme target

His tag

eIF4E1 

deIF4E

enzyme target

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)