GstS1 is stably associated with pelleted thin filaments of indirect flight muscles, but upon digestion of Tm1 with Igase, GstS1 is released into free solution.

Source was dissected, demembranted indirect flight muscle of wild-type fly line; proteins produced from endogenous genes.

Purified indirect flight muscles were digested with Igase, an endoproteinase that is predicted to cleave only Tm1 protein.

Source was female adult thorax of Oregon-R fly line

Diagonal gels were performed by running a non-reducing dimension in a tube gel and a reducing second dimension in a slab gel. Under non-reducing conditions the disulfide bridge containing complexes appear delayed, but after the reducing second dimension these proteins will appear off the theoretical diagonal, given by a straight line from the origin of the gel and the signal from the monomeric forms (GstS1 and Tm1). The MAC144 antibody clearly detected heterodimers of Tm1 with GstS1.