FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tut, bgcn
FlyBase ID
FBig0000103227
Interaction Network
Interactions Browser links
tut network
bgcn network
Reported Interactions
FBrf0226802-10.coIP.WB
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
bgcn 

Bgcn

bait

FLAG tag, Myc tag

tut 

Tut

prey

TAP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Myc-tagged bgcn immunoprecipitated from TAP-tut complexes purified from cells expressing tagged tut, bgcn and bam.

(Chen et al., 2014)

Sequential TAP-tut and Myc-bgcn purifications indicate that tut, bgcn and bam co-exist in the same complex. Given that direct tut-bgcn interactions are not observed in yeast two hybrid assays, bam must bridge tut and bgcn. This complex is not RNA-dependent (resistant to RNaseA treatment).

(Chen et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)
FBrf0226802-12.coIP.WB
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tut 

Tut

bait

TAP tag

bgcn 

Bgcn

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult male testis of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Chen et al., 2014)
FBrf0226802-15.coIP.WB
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
bgcn 

Bgcn

bait

FLAG tag, Myc tag

tut 

Tut

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)
FBrf0226802-9.coIP.WB
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tut 

Tut

bait

TAP tag

bgcn 

Bgcn

prey

FLAG tag, Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cells were transfected with tagged tut, bgcn and bam.

(Chen et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)