FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Drice, Diap1
FlyBase ID
FBig0000103229
Interaction Network
Interactions Browser links
Drice network
Diap1 network
Reported Interactions
FBrf0174987-1.ENZ.MW
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drice 

DrICE

enzyme
Drice 

DrICE

enzyme
Diap1 

DIAP1

enzyme target
Diap1 

DIAP1

enzyme target
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0174987-3.CH.MW
Reference
Yan et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drice 

DrICE

neutral component
Drice 

DrICE

neutral component
Drice 

DrICE

neutral component
Diap1 

DIAP1

neutral component
Diap1 
Diap1 
Diap1 
Diap1 

DIAP1

neutral component
Diap1 

DIAP1

neutral component
Diap1 
Diap1 
Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
BIR1 domain
sufficient binding region
aa 21-145

coordinates relative to Diap1-PA

Diap1 
BIR1 domain residues 77,79
mutation disrupting interaction
aa 77,79

K77D,K79D, coordinates relative to Diap1-PA

Diap1 
BIR1 domain residues 94,99
mutation disrupting interaction
aa 94,99

D94K,E99K, coordinates relative to Diap1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Addition of a 10 aa hid N-terminal peptide disrupts the Diap1-Drice interaction except with versions of the Diap1 BIR1 domain carrying a G88S substitution. The presence of the Diap1 auto-inhibitory N-terminus disrupts the Diap1-Drice interaction.

(Yan et al., 2004)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Yan et al., 2004)
FBrf0184187-1.PD.WB
Reference
Tenev et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Diap1 

DIAP1

bait

GST tag

Diap1 
Diap1 
Diap1 
Drice 

drICE

prey

V5 tag

Drice 
Drice 
Drice 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
N-terminal region
sufficient binding region
aa 1-146

coordinates relative to Diap1-PA

Diap1 
N-terminal region residue P55
mutation disrupting interaction
aa 55

P55L, coordinates relative to Diap1-PA

Diap1 
N-terminal region residue C80
mutation disrupting interaction
aa 80

C80G, coordinates relative to Diap1-PA

Drice 
IBM motif
necessary binding region
ALG
Drice 
IBM motif residue 29
mutation disrupting interaction
aa 29

A29V, coordinates relative to Drice-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected cell extract.

(Tenev et al., 2005)
FBrf0192581-1.coIP.WB
Reference
Leulier et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Diap1 

DIAP1

bait

TAP tag

Drice 

drICE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Leulier et al., 2006)
FBrf0206305-2.coIP.WB
Reference
Ditzel et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drice 

drICE

bait

V5 tag

Diap1 

DIAP1

prey
Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
N-terminal domain
mutation increasing interaction
aa 1-20

deletion of N-terminus

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ditzel et al., 2008)
FBrf0226660-4.coIP.WB
Reference
Huang et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Drice 

DrICE

bait

Myc tag

Diap1 

DIAP1

prey

V5 tag

Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
ΔN form
sufficient binding region
aa 21-438

mimics the caspase cleaved form

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Huang et al., 2014)

The Drice-Diap1 interaction is stronger when Ubr3 is co-expressed.

(Huang et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)