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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103229
Interaction Network
Interactions Browser links
Diap1 network
Drice network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0174987-1.ENZ.MW
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DrICE

enzyme

DrICE

enzyme

DIAP1

enzyme target

DIAP1

enzyme target
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0174987-3.CH.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DrICE

neutral component

DrICE

neutral component

DrICE

neutral component

DIAP1

neutral component

DIAP1

neutral component

DIAP1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BIR1 domain
sufficient binding region
aa 21-145

coordinates relative to Diap1-PA

BIR1 domain residues 77,79
mutation disrupting interaction
aa 77,79

K77D,K79D, coordinates relative to Diap1-PA

BIR1 domain residues 94,99
mutation disrupting interaction
aa 94,99

D94K,E99K, coordinates relative to Diap1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Addition of a 10 aa hid N-terminal peptide disrupts the Diap1-Drice interaction except with versions of the Diap1 BIR1 domain carrying a G88S substitution. The presence of the Diap1 auto-inhibitory N-terminus disrupts the Diap1-Drice interaction.

FBrf0184187-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait

GST tag

drICE

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N-terminal region
sufficient binding region
aa 1-146

coordinates relative to Diap1-PA

N-terminal region residue P55
mutation disrupting interaction
aa 55

P55L, coordinates relative to Diap1-PA

N-terminal region residue C80
mutation disrupting interaction
aa 80

C80G, coordinates relative to Diap1-PA

IBM motif
necessary binding region
ALG
IBM motif residue 29
mutation disrupting interaction
aa 29

A29V, coordinates relative to Drice-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected cell extract.

FBrf0192581-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait

TAP tag

drICE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0206305-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

drICE

bait

V5 tag

DIAP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N-terminal domain
mutation increasing interaction
aa 1-20

deletion of N-terminus

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0226660-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DrICE

bait

Myc tag

DIAP1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ΔN form
sufficient binding region
aa 21-438

mimics the caspase cleaved form

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

The Drice-Diap1 interaction is stronger when Ubr3 is co-expressed.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)