FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
TP53INP, EcR
FlyBase ID
FBig0000103258
Interaction Network
Interactions Browser links
TP53INP network
EcR network
Reported Interactions
FBrf0212109-1.coIP.WB
Reference
Francis et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
TP53INP 

dDOR

bait

FLAG tag

TP53INP 
TP53INP 
EcR 

EcR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
TP53INP 
FENLL motif
mutation decreasing interaction
aa 276-280

FENLL mutated to AENAA, coordinates relative to DOR-PG

TP53INP 
FENLL motif
necessary binding region
YVTPPPCFTSIGPINMETSPFENLLIEHP

characteristic of EcR-binding isoforms

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

S2 cells were also co-transfected with usp.

(Francis et al., 2010)

Only the long isoform of DOR containing the FENLL motif bound EcR (DOR-PG, -PH). Other DOR isoforms lacking the FENLL motif do not bind EcR.

(Francis et al., 2010)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Francis et al., 2010)
FBrf0212109-2.PD.WB
Reference
Francis et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait

GST tag

TP53INP 

dDOR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transfected S2 cells.

(Francis et al., 2010)

DOR did not interact with usp, Hr46, or ftz-f1 in this GST pulldown experiment.

(Francis et al., 2010)
FBrf0229176-20a.coIP.MS
Reference
Xie et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage P1
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait
TP53INP 

dDOR

prey

1 peptide identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was white prepupae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Xie et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)