FB2026_02 , released June 18, 2026
Gene: Dmel\TP53INP
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General Information
Symbol
Dmel\TP53INP
Species
D. melanogaster
Name
Tumor protein p53 inducible nuclear protein
Annotation Symbol
CG11347
Feature Type
protein_coding_gene
FlyBase ID
FBgn0035542
Gene Model Status
Current
Stock Availability
17 publicly available
Gene Summary
Tumor protein p53 inducible nuclear protein (TP53INP) encodes a transcriptional coactivator of ecdysone receptor signaling required during metamorphosis. It is antagonized by insulin signaling via the product of foxo in the fat body and forms part of a feed-forward mechanism whereby ecdysone potentiates its own signaling. The product of TP53INP also regulates autophagosome formation and protein degradation via ecdysone signaling. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

DOR, dDOR

Key Links
Genomic Location
Cytogenetic map
64B4-64B5
Sequence location
Recombination map
3-12
RefSeq locus
NT_037436 REGION:4406943..4420648
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables nuclear receptor binding
inferred from physical interaction with FLYBASE:EcR; FB:FBgn0000546
(Francis et al., 2010)
enables transcription coactivator activity
inferred from direct assay
(Francis et al., 2010)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in ecdysone receptor signaling pathway
inferred from mutant phenotype
(Francis et al., 2010)
involved_in positive regulation of ecdysone receptor signaling pathway
inferred from mutant phenotype
(Francis et al., 2010)
involved_in positive regulation of transcription by RNA polymerase II
inferred from direct assay
(Francis et al., 2010)
involved_in regulation of autophagy
inferred from mutant phenotype
(Mauvezin et al., 2010)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in autophagosome assembly
inferred from biological aspect of ancestor with PANTHER:PTN001270888
(GO Reference Genome Project, 2011-)
involved_in positive regulation of DNA-templated transcription
inferred from biological aspect of ancestor with PANTHER:PTN001270888
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in autophagosome
inferred from biological aspect of ancestor with PANTHER:PTN001270888
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN001270888
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred by curator from GO:0003713
(Francis et al., 2010)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Tumor protein p53 inducible nuclear protein (TP53INP) encodes a transcriptional coactivator of ecdysone receptor signaling required during metamorphosis. It is antagonized by insulin signaling via the product of foxo in the fat body and forms part of a feed-forward mechanism whereby ecdysone potentiates its own signaling. The product of TP53INP also regulates autophagosome formation and protein degradation via ecdysone signaling. [Date last reviewed: 2019-03-07]
Summary (Interactive Fly)

coactivator of ecdysone receptor needed during metamorphosis - antagonized by insulin signaling via FOXO in the fat body - part of a feed-forward mechanism whereby ecdysone potentiates its own signaling - regulates autophagosome formation and protein degradation

Gene Model and Products
Number of Transcripts
8
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\TP53INP for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry M9NFM7)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
TP53INP-RA
FBtr0073296
NM_139643
2022
387
TP53INP-RB
FBtr0073295
NM_168083
2311
387
TP53INP-RC
FBtr0073297
NM_168085
1040
273
TP53INP-RD
FBtr0073294
NM_168084
2450
387
TP53INP-RE
FBtr0113140
NM_001104033
1906
387
TP53INP-RF
FBtr0113141
NM_001104034
1255
229
TP53INP-RG
FBtr0308033
NM_001259695
2158
417
TP53INP-RH
FBtr0308034
NM_001259696
2019
417
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
TP53INP-PA
FBpp0073152
42.3
387
8.06
Q9VZC7
NP_647900
AAF47897
TP53INP-PB
FBpp0073151
42.3
387
8.06
Q9VZC7
NP_728990
AAF47898
TP53INP-PC
FBpp0073153
30.2
273
9.09
Q8IRB4
NP_728992
AAN11602
TP53INP-PD
FBpp0073150
42.3
387
8.06
Q9VZC7
NP_728991
AAN11601
TP53INP-PE
FBpp0112053
42.3
387
8.06
Q9VZC7
NP_001097503
ABW08462
TP53INP-PF
FBpp0112054
25.6
229
9.94
A8JNK7
NP_001097504
ABW08463
TP53INP-PG
FBpp0300394
45.7
417
7.14
M9NFM7
NP_001246624
AFH04295
TP53INP-PH
FBpp0300395
45.7
417
7.14
M9NFM7
NP_001246625
AFH04296
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

387 aa isoforms: TP53INP-PA, TP53INP-PB, TP53INP-PD, TP53INP-PE
417 aa isoforms: TP53INP-PG, TP53INP-PH
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TP53INP using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.82

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
ventral midline of embryo
(Wheeler et al., 2009)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
yolk nucleus
(Fisher et al., 2012)
embryonic stage 7 -- 8
amnioserosa anlage
(Fisher et al., 2012)
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
amnioserosa
(Fisher et al., 2012)
amnioserosa primordium
(Fisher et al., 2012)
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
amnioserosa
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
larval lateral VNC glial cell
(Fisher et al., 2012)
larval ventral midline glial cell
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
trunk visceral muscle primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
amnioserosa
(Fisher et al., 2012)
dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)
embryonic brain
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic head epidermis
(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
embryonic ventral epidermis
(Fisher et al., 2012)
larval muscle system
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
larval visceral muscle cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
larval ventral midline glial cell
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
ventral midline of embryo
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
ventral sensory complex primordium
(Fisher et al., 2012)
embryonic stage 15
ventral nerve cord
(Magalhaes et al., 2007)
embryonic brain | restricted
(Magalhaes et al., 2007)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
(Francis et al., 2010)
prepupal stage
embryonic/larval fat body
(Francis et al., 2010)
adult stage
(Francis et al., 2010)
Additional Descriptive Data

The relative abundance of the different protein isoforms was assayed. The "long" form is most abundant. The "FENLL" form, which has an extra domain due to an alternative splice, is generally at about half the level of the long form and the short form is expressed at low levels. In fat body of early pupae, The FENLL form predominates.

(Francis et al., 2010)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\TP53INP in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of TP53INP
Transgenic constructs containing regulatory region of TP53INP
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3L)Exel6101
(Ryder, 2004.4.26)
Inferred from location ( 8 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\TP53INP1
7 of 14
Yes
Yes
Hsap\TP53INP2
6 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Tp53inp1
6 of 14
Yes
Yes
Rnor\Tp53inp2
4 of 14
No
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Trp53inp1
6 of 14
Yes
Yes
Mmus\Trp53inp2
4 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\tp53inp1
4 of 13
Yes
Yes
Xtro\tp53inp2
4 of 13
Yes
Yes
Danio rerio (Zebrafish) (4)
Drer\tp53inp1
6 of 14
Yes
Yes
Drer\tp53inp2
2 of 14
No
Yes
Drer\si:ch211-260e23.9
1 of 14
No
Yes
Drer\tp53inp2
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (0)
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:TP53INP. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    TP53INP1; tumor protein p53 inducible nuclear protein 1
    7 of 14
    606185
        TP53INP2; tumor protein p53 inducible nuclear protein 2
        6 of 14
        617549
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            park
            suppressible
            TP53INP
            (Dinh et al., 2021)
            Pink1
            suppressible
            TP53INP
            (Dinh et al., 2021)
            Pink1
            enhanceable
            TP53INP
            (Dinh et al., 2021)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3L
            Recombination map
            3-12
            Cytogenetic map
            64B4-64B5
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            64B4-64B5
            Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            3-12

            (FlyBase, 2016)

            3-9.7

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (17)
            Genomic Clones (17)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (77)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments
                Relationship to Other Genes
                Source for database merge of
                Additional comments
                Nomenclature History
                Source for database identify of

                Source for identity of: DOR CG11347

                (Francis et al., 2010)

                Source for identity of: TP53INP DOR

                (Teleman, 2022.7.19)
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (7)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 65 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                References (51)