FlyBase Allele Report: Dmel\TP53INP[KO]

General Information

Symbol

Dmel\TP53INP^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0260615

Feature type

allele

Associated gene

Dmel\TP53INP

Associated Insertion(s)

TI{TI}TP53INP^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Francis et al., 2010)

Mutagen

gene targeting by homologous recombination

(Francis et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}TP53INP^KO

Cytology

Description

Sequences encoding the entire open reading frame of all DOR splice variants have been replaced with a mini-w marker.

(Francis et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 6

modeled by Pink1^B9

(Dinh et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage

(Francis et al., 2010)

some die during P-stage

(Francis et al., 2010)

Phenotype Manifest In

anterior pupal spiracle

(Francis et al., 2010)

embryonic/larval fat body | third instar larval stage

(Dinh et al., 2021)

lysosome | decreased number | third instar larval stage

(Dinh et al., 2021)

salivary gland

(Francis et al., 2010)

Detailed Description

Statement

Reference

DOR^KO homozygotes display smaller and fewer lysosomes (LysoTracker puncta) in the third instar larval fat body.

(Dinh et al., 2021)

Mutant larvae have no obvious defects and pupate at almost the same time as control larvae. Only 59% of mutant animals eclose as adults. 35% of mutant pupae show impaired anterior spiracle eversion. Destruction of the larval salivary glands during metamorphosis is delayed in 66% of mutant pupae.

Feeding with 20-hydroxyecdysone significantly rescues the lethality of mutant animals.

Mutant adults have reduced total body triglyceride levels, increased glycogen levels and increased circulating trehalose levels (normalised to total body protein) compared to controls.

(Francis et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

TP53INP^KO/TP53INP^KO is an enhancer of mitochondrion | adult stage phenotype of Pink1^B9

(Dinh et al., 2021)

TP53INP^KO/TP53INP^KO is an enhancer of mitochondrial membrane | adult stage phenotype of Pink1^B9

(Dinh et al., 2021)

TP53INP^KO/TP53INP^KO is an enhancer of dorsal longitudinal indirect flight muscle cell | adult stage phenotype of Pink1^B9

(Dinh et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Pink1[B9]/Y, DOR^KO/DOR^KO males show flight muscle degeneration and disorganization visible at the macroscopic scale: the extremities of dorsal longitudinal flight muscles appear ripped off from the cuticle and the muscle fibers are disorganized and torn

(Dinh et al., 2021)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

TP53INP^KO is rescued by TP53INP^FENLL.UAS/Scer\GAL4^Tub.PU

(Francis et al., 2010)

Not rescued by

TP53INP^KO is not rescued by Scer\GAL4^Tub.PU/TP53INP^long.UAS

(Francis et al., 2010)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76338

w¹¹¹⁸; TI{TI}TP53INP^KO/TM3, P{ActGFP}JMR2, Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DOR^KO

TP53INP^KO

Name Synonyms

dDOR knockout

(Francis et al., 2010)

Secondary FlyBase IDs

References (2)

Report Sections

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