Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

fz binds Patj, but not par-6, aPKC, dlg1, sdt or baz in this assay. fz2 does not interact with Patj.

Two-hybrid system: yeast

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Patj binds GST-fz, but not GST-fz2.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Patj coimmunoprecipitates with GFP-fz, but not with GFP-fz2.