FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103440
Interaction Network
Interactions Browser links
egr network
wgn network
Reported Interactions
FBrf0151360-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egr 

Eiger

bait

HA tag

egr 
wgn 

Wengen

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egr 
TNF homology domain
necessary binding region
aa 272-409
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0151360-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wgn 

Wengen

bait

FLAG tag

wgn 
wgn 
egr 

Eiger

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wgn 
extracellular and transmembrane domains
sufficient binding region
aa 1-224

coordinates relative to wgn-PA

wgn 
TNFR homology domain
necessary binding region
aa 100-137

coordinates relative to wgn-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0248618-2.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egr 

Egr

neutral component
egr 

Egr

neutral component
egr 
egr 

Egr

neutral component
egr 
wgn 

Wgn

neutral component
wgn 
wgn 

Wgn

neutral component
wgn 

Wgn

neutral component
wgn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egr 
extracellular domain
sufficient binding region
aa 146-409
wgn 
extracellular domain
sufficient binding region
aa 78-201
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

FBrf0248618-5.SLS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egr 

Egr

neutral component
egr 
egr 

Egr

neutral component
egr 
egr 

Egr

neutral component
wgn 
wgn 

Wgn

neutral component
wgn 

Wgn

neutral component
wgn 
wgn 

Wgn

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egr 
extracellular domain
sufficient binding region
aa 146-409
wgn 
extracellular domain
sufficient binding region
aa 78-201
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

The wgn extracellular domain bound to the egr extracellular domain is a hetero-hexamer in solution.

FBrf0248618-7.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egr 

Egr

bait
egr 
wgn 

Wgn

prey
wgn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egr 
extracellular domain
sufficient binding region
aa 146-409
wgn 
extracellular domain
sufficient binding region
aa 78-201
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)