FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hsp83, InR
FlyBase ID
FBig0000103592
Interaction Network
Interactions Browser links
Hsp83 network
InR network
Reported Interactions
FBrf0224702-234.coIP.MS
Reference
Friedman et al., 2011
Description
physical association
Assay
Collection
RTK-Ras-ERK_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
InR 

InR

bait

TAP tag

Hsp83 

Hsp83

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Friedman et al., 2011)

Overall SAINT probability of interaction = 0.98.

(Friedman et al., 2011)
FBrf0240343-1.PLA
Reference
Huang and Wang, 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hsp83 

Hsp83

neutral component

Myc tag

Hsp83 

Hsp83

neutral component

Myc tag

Hsp83 

Hsp83

neutral component

Myc tag

InR 

InR

neutral component

HA tag

InR 
InR 

InR

neutral component

HA tag

InR 

InR

neutral component

HA tag

InR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
InR 
intracellular domain
sufficient binding region
aa 1372-2144

coordinates relative to InR-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Huang and Wang, 2018)
FBrf0240343-4.BiFC.FM
Reference
Huang and Wang, 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hsp83 

Hsp83

bait

YFP tag, N-terminal fragment

Hsp83 
InR 

InR

prey

YFP tag, C-terminal fragment

InR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
InR 
intracellular domain
sufficient binding region
aa 1372-2144

coordinates relative to InR-PA

Hsp83 
N-terminal domain
necessary binding region
aa 1-537
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Huang and Wang, 2018)
FBrf0240343-7.BiFC.FM
Reference
Huang and Wang, 2018
Description
physical association
Assay
Collection
Source/Stage
neuroblast / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hsp83 

Hsp83

bait

YFP tag, N-terminal fragment

InR 

InR

prey

YFP tag, C-terminal fragment

InR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
InR 
intracellular domain
sufficient binding region
aa 1372-2144

coordinates relative to InR-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Huang and Wang, 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)