Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

CG9590 interacts weakly with GTP-locked Rab14 (and more strongly, with GTP-locked Rab2), but neither form of the other 15 Rabs tested.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

CG9590 specifically binds the GTP-bound state of Rab2 or Rab14 (not GDP-locked forms).