Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Positive control.

Two-hybrid system: yeast GAL4-BD/VP16-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The Apc2 SAMP2 domain binds Axn much more strongly than the Apc2 SAMP1 domain, but full binding of Apc2 to Axn is observed when both SAMP domains are present.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Positive control.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Source was cell extract of SW480 cell line; bait produced from transfected construct; prey produced from transfected construct.

In the context of full length Apc2, the C-terminal SAMP domains are necessary for binding to Axn. However, deletion of SAMP domains along with deletion of either the Apc2 "20-amino-acid repeat 2" or "conserved sequence B" regions restores binding to Axn. This suggest that binding to Axn mediated by the N-terminal Arm repeats in Apc2 is inhibitied by the "20-amino-acid repeat 2" and "conserved sequence B" regions.

BiFC signal was viewed only in regions of high wg signaling and may represent an inactive destruction complex.

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.