Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-DBD/VP16-AD

The ToRC complex consists of tou, Iswi and CtBP. ToRC is an ATP-dependent chromatin assembly factor.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

tou coimmunoprecipitates Iswi in the presence of absense of CtBP. No interaction was detected between Iswi and CtBP in this assay, suggesting that tou bridges Iswi and CtBP in the ToRC complex. The ToRC complex is an ATP-dependent chromatin assembly factor.

tou, Iswi and CtBP copurify as the ToRC complex, with apparent molecular weight of about 500 kDa, after three chromatographic steps and a glycerol gradient.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.