Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

tou coimmunoprecipitates CtBP in the presence of absense of Iswi. No interaction was detected between Iswi and CtBP in this assay, suggesting that tou bridges Iswi and CtBP in the ToRC complex. The ToRC complex is an ATP-dependent chromatin assembly factor.

The ToRC complex consists of tou, Iswi and CtBP. ToRC is an ATP-dependent chromatin assembly factor.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

tou, Iswi and CtBP copurify as the ToRC complex, with apparent molecular weight of about 500 kDa, after three chromatographic steps and a glycerol gradient. Additional CtBP-free tou-Iswi complex can be detected.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.