FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nito, Sxl
FlyBase ID
FBig0000103763
Interaction Network
Interactions Browser links
nito network
Sxl network
Reported Interactions
FBrf0229644-1.coIP.WB
Reference
Yan and Perrimon, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
nito 

Nito

bait

GFP tag

Sxl 

Sxl

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yan and Perrimon, 2015)

The nito-Sxl interaction is disrupted by RNase treatment. nito interacts with both Sxl RNA and protein.

(Yan and Perrimon, 2015)
FBrf0229644-2.coIP.WB
Reference
Yan and Perrimon, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait

HA tag

nito 

Nito

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yan and Perrimon, 2015)
FBrf0229644-3.coIP.PCR
Reference
Yan and Perrimon, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
nito 

Nito

bait

GFP tag

Sxl 

Sxl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Yan and Perrimon, 2015)

nito interacts with both Sxl RNA and protein.

(Yan and Perrimon, 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)