FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
crb, aPKC
FlyBase ID
FBig0000103789
Interaction Network
Interactions Browser links
crb network
aPKC network
Reported Interactions
FBrf0180375-1.PD.WB
Reference
Sotillos et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

GST tag

crb 
crb 
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
RNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI
crb 
intracellular domain
necessary binding region
ERLI
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Sotillos et al., 2004)
FBrf0180375-3.PD.WB
Reference
Sotillos et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait

His tag

crb 

Crb

prey

GST tag

crb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
RNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sotillos et al., 2004)
FBrf0191891-10.PBD.FM
Reference
Kempkens et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

fused to VSV-G protein

crb 
aPKC 

DaPKC

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
aa 2079-2146

coordinates relative to crb-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fixed S2 cells; bait produced from transfected construct; prey produced from endogenous gene.

(Kempkens et al., 2006)

Bait protein was fused to an VSV-G protein, which targets the chimera to the plasma membrane. The ability of this chimera to recruit prey protein to plasma membrane was taken as evidence of direct physical interaction.

(Kempkens et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)