FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
WASp, Abi
FlyBase ID
FBig0000103790
Interaction Network
Interactions Browser links
WASp network
Abi network
Reported Interactions
FBrf0162072-2.Y2H
Reference
Bogdan and Klambt, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
WASp 

WASP

bait

fused to GAL4 DNA-binding domain

Abi 

Abi

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bogdan and Klambt, 2003)

Abi interacts with Hem (kette) and WASp, but no direct interaction between Hem and WASp was observed.

(Bogdan and Klambt, 2003)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bogdan and Klambt, 2003)
FBrf0188146-3.Y2H
Reference
Bogdan et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

unspecified role
Abi 

Abi

unspecified role
Abi 
Abi 
Abi 

Abi

unspecified role
Abi 
Abi 
WASp 

WASP

unspecified role
WASp 

WASP

unspecified role
WASp 

WASP

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abi 
SH3 domain
necessary binding region
Abi 
SH3 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bogdan et al., 2005)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Bogdan et al., 2005)
FBrf0188146-4.PD.WB
Reference
Bogdan et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
WASp 

WASP

bait

GST tag

Abi 

Abi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2R+ cell extract.

(Bogdan et al., 2005)
FBrf0188146-9.coIP.WB
Reference
Bogdan et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait
WASp 

WASP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bogdan et al., 2005)
FBrf0216499-4.CH.WB
Reference
Stephan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

neutral component
Abi 

Abi

neutral component
Abi 

Abi

neutral component
WASp 

WASP

neutral component
WASp 

WASP

neutral component
WASp 

WASP

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins derived from adult head extract.

(Stephan et al., 2011)

A small fraction of Abi cofractionates with WASp in protein complexes of about 200-300 kDa. Little SCAR or Hem is found in these fractions.

(Stephan et al., 2011)
FBrf0242076-3.PD.WB
Reference
Kim et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait

GST tag

Abi 
Abi 
WASp 

WASp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abi 
SH3 domain
sufficient binding region
aa 388-473

coordinates relative to Abi-PA

Abi 
SH3 domain residue 452
mutation disrupting interaction
aa 452

W452K, coordinates relative to Abi-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ cell extract.

(Kim et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)