FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Tob, orb2
FlyBase ID
FBig0000103843
Interaction Network
Interactions Browser links
Tob network
orb2 network
Reported Interactions
FBrf0224133-14.coIP.WB
Reference
White-Grindley et al., 2014
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tob 

Tob

bait
orb2 

Orb2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tob coimmunoprecipitates both the short "A" and long "B" isoforms of orb2, as well as orb2 oligomers.

(White-Grindley et al., 2014)

Overexpression of Tob stabilizes the orb2 "A" isoform, and consequently increases the level of orb2 oligomer.

(White-Grindley et al., 2014)

Neuronal stimulation increases the interaction of Tob with orb2.

(White-Grindley et al., 2014)

Source was adult heads of wild type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(White-Grindley et al., 2014)
FBrf0224133-18.coIP.WB
Reference
White-Grindley et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
orb2 

Orb2

bait
Tob 

Tob

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(White-Grindley et al., 2014)
FBrf0224133-26.coIP.MS
Reference
White-Grindley et al., 2014
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
orb2 

Orb2

bait

HA tag

Tob 

Tob

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(White-Grindley et al., 2014)
FBrf0224133-3.coIP.WB
Reference
White-Grindley et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Tob 

Tob

bait

FLAG tag

orb2 

Orb2A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tob binds both the "A" and "B" isoforms of orb2, as well as orb2 oligomers.

(White-Grindley et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(White-Grindley et al., 2014)
FBrf0224133-41.PD.A
Reference
White-Grindley et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tob 

Tob

bait

MBP tag

orb2 

Orb2

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(White-Grindley et al., 2014)
FBrf0224133-42.coIP.WB
Reference
White-Grindley et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
orb2 

Orb2

bait

HA tag

Tob 

Tob

prey

FLAG tag

Tob 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tob 
Orb2 interacting domain
necessary binding region
aa 245-262

coordinates relative to Tob-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(White-Grindley et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)