Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

Tagged proteins expressed in embryonic neurons only.

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

Positive control.