FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO3, tud
FlyBase ID
FBig0000103907
Interaction Network
Interactions Browser links
AGO3 network
tud network
Reported Interactions
FBrf0209532-11.CS.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tud

neutral component
tud 

Tud

neutral component
tud 

Tud

neutral component
AGO3 

AGO3

neutral component
AGO3 

AGO3

neutral component
AGO3 

AGO3

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)
FBrf0209532-4.coIP.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tud

bait
AGO3 

AGO3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)
FBrf0209532-5.PD.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

bait

biotin tag

AGO3 
tud 

Tud

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO3 
interaction domain
sufficient binding region
aa 58-81

coordinates relative to AGO3-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide commercially synthesized; prey derived from wild-type ovary extract.

(Nishida et al., 2009)
FBrf0209532-9.coIP.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

bait
tud 

Tud

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)
FBrf0229343-6.coIP.WB
Reference
Sato et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

bait
tud 

Tud

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

tud in ovary lysate interacts with sDMA-modified AGO3 but not non-sDMA-modified AGO3.

(Sato et al., 2015)

Source was ovaries of Oregon-R fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sato et al., 2015)
FBrf0229343-7.coIP.WB
Reference
Sato et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tud

bait
AGO3 

AGO3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

tud does not interact with AGO3 in krimp mutant flies.

(Sato et al., 2015)

Source was ovaries of Oregon-R fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sato et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)