FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Jra, Sumo
FlyBase ID
FBig0000103919
Interaction Network
Interactions Browser links
Jra network
Sumo network
Reported Interactions
FBrf0229794-19.PD.WB
Reference
Handu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Jra 

jra

bait

GST tag

Sumo 

SUMO

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vivo; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Handu et al., 2015)
FBrf0229794-20.PD.WB
Reference
Handu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

His tag

Jra 

jra

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vivo; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Handu et al., 2015)
FBrf0229794-21.coIP.WB
Reference
Handu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

His, FLAG tag

Jra 

jra

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 529SU cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Handu et al., 2015)
FBrf0252954-1.PD.WB
Reference
Soory and Ratnaparkhi, 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Jra 

Jra

bait

GST tag

Jra 
Sumo 

Sumo

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Jra 
NDSM and SCSM conjugation motifs residues 29 and 190
necessary binding region
aa 29 and 190

K29R and K190R.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Soory and Ratnaparkhi, 2022)

Drosophila Sumo enzyme cycle components expressed in a bacterial system with bait and prey.

(Soory and Ratnaparkhi, 2022)
FBrf0252954-2.PD.WB
Reference
Soory and Ratnaparkhi, 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Jra 

Jra

bait

HIS tag

Jra 
Sumo 

Sumo

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Jra 
NDSM conjugation motif residue 29
necessary binding region
aa 29

K29R.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Soory and Ratnaparkhi, 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)