FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdc42, Whamy
FlyBase ID
FBig0000103965
Interaction Network
Interactions Browser links
Cdc42 network
Whamy network
Reported Interactions
FBrf0208460-3.PD.A
Reference
Liu et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Cdc42

bait

GST tag

Whamy 

Whamy

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Liu et al., 2009)
FBrf0230860-1.PD.WB
Reference
Brinkmann et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Cdc42

bait

GST tag

Whamy 

WHAMY

prey

EGFP tag

Whamy 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Whamy 
CRIB domains 1 and 2
necessary binding region
aa 1-254

coordinates relative to Whamy-PD, "ΔCRIB1+2" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ extract.

(Brinkmann et al., 2016)

Whamy binds to both GDP- and GTP-bound Cdc42. Only one of the two Whamy CRIB domains is required for binding, but deletion of both CRIB domains abolishes the interaction.

(Brinkmann et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)