Ric-8
Pins
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Coimmunoprecipitation experiments in a Gαi mutant background show that ric8a can complex with pins in the absence of Gαi.
MBP tag
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.