FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
inaC, inaD
FlyBase ID
FBig0000103990
Interaction Network
Interactions Browser links
inaC network
inaD network
Reported Interactions
FBrf0096480-3.coIP.WB
Reference
Tsunoda et al., 1997
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

inaD

bait
inaC 

eye-PKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was membranes from adult heads of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tsunoda et al., 1997)

trp, norpA and inaC, but not ninaE or Gαq, coimmunoprecipitate with inaD.

(Tsunoda et al., 1997)
FBrf0096480-5.PD.WB
Reference
Tsunoda et al., 1997
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

GST tag

inaD 
inaC 

eye-PKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ4 domain
sufficient binding region
aa 485-577

coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Tsunoda et al., 1997)
FBrf0103225-1.Y2H
Reference
Adamski et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaC 

eye-PKC

bait

fused to LexA DNA-binding domain

inaC 
inaC 
inaD 

INAD

prey

fused to B42 activation domain

inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaC 
C-terminus
sufficient binding region
TII
inaC 
C-terminus residue I700
mutation disrupting interaction
aa 700

I700D or I700N

inaD 
PDZ2 domain
sufficient binding region
aa 206-365
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Adamski et al., 1998)

Two-hybrid system: yeast LexA-BD/B42-AD

(Adamski et al., 1998)
FBrf0103225-2.FW.A
Reference
Adamski et al., 1998
Description
physical association
Assay
Collection
Source/Stage
retina / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaC 

eye-PKC

bait
inaD 

inaD

prey

[35]S label

inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ2 domain
sufficient binding region
aa 206-365
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from adult retinal extract; prey produced and labeled by in vitro translation..

(Adamski et al., 1998)
FBrf0103542-7.coIP.WB
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

Myc tag

inaD 
inaD 
inaC 

PKC

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ3L domain
sufficient binding region
aa 346-489

coordinates relative to inaD-PA

inaD 
PDZ4 domain
sufficient binding region
aa 476-581

coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 1998)
FBrf0103542-8.PD.A
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

GST tag

inaC 

PKC

prey

[35]S label

inaC 
inaC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaC 
catalytic domain
sufficient binding region
aa 472-700

coordinates relative to inaC-PA

inaC 
catalytic domain
necessary binding region
aa 370-700

coordinates relative to inaC-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Xu et al., 1998)
FBrf0127205-2.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaC 

eye-PKC

enzyme
inaC 

eye-PKC

enzyme
inaD 

INAD

enzyme target

P[32] label

inaD 

INAD

enzyme target

P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0132378-2.coIP.WB
Reference
Tsunoda et al., 2001
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait
inaC 

PKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head membrane extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tsunoda et al., 2001)

Using heat shock constructs in an inaD mutant background, the authors show that inaD interacts with norpA and inaC shortly after heat shock induced expression (3hr), long before these proteins reach the rhabdomeres and long before light

(Tsunoda et al., 2001)

responses have recovered. This suggests that the complex is preassembled before transport.

(Tsunoda et al., 2001)
FBrf0136982-1.PD.A
Reference
Kumar and Shieh, 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

GST tag

inaD 
inaC 

PKC

prey

[35]S label

inaC 
inaC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ2 domain
sufficient binding region
aa 206-363

coordinates relative to inaD-PA

inaC 
C-terminal region
sufficient binding region
aa 562-700

coordinates relative to inaC-PA

inaC 
C-terminal -3 Ile residue
mutation disrupting interaction
aa 697

I697E or I697K, coordinates relative to inaC-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kumar and Shieh, 2001)
FBrf0136982-2.PD.A
Reference
Kumar and Shieh, 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaC 

PKC

bait

GST tag

inaC 
inaD 

INAD

prey

[35]S label

inaD 
inaD 
inaD 
inaD 
inaD 
inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ2 domain
sufficient binding region
aa 206-363

strong binding, coordinates relative to inaD-PA

inaD 
PDZ4 domain
sufficient binding region
aa 485-577

weak binding, coordinates relative to inaD-PA

inaC 
C-terminal region
sufficient binding region
aa 562-700

coordinates relative to inaC-PA

inaD 
PDZ2 residue H310
mutation decreasing interaction
aa 310

H310R, coordinates relative to inaD-PA

inaD 
PDZ2 residue H310
mutation increasing interaction
aa 310

H310L, coordinates relative to inaD-PA

inaD 
PDZ2 residue N319
mutation increasing interaction
aa 319

N319S, naturally occuring variant, coordinates relative to inaD-PA

inaD 
PDZ2 residue P282
mutation increasing interaction
aa 282

P282L, naturally occuring variant, coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kumar and Shieh, 2001)
FBrf0192668-1.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaC 

eye-PKC

enzyme
inaC 

eye-PKC

enzyme
inaD 

INAD

enzyme target

GST tag, P[32] label

inaD 

INAD

enzyme target

GST tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)