FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Fmr1, piwi
FlyBase ID
FBig0000103996
Interaction Network
Interactions Browser links
Fmr1 network
piwi network
Reported Interactions
FBrf0191897-4.coIP.WB
Reference
Megosh et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
piwi 

piwi

bait

TAP tag

Fmr1 

dFMRP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Megosh et al., 2006)
FBrf0230867-1.coIP.WB
Reference
Jiang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMRP

bait
piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Fmr1-piwi interaction is RNA-independent as it persists after RNase treatment.

(Jiang et al., 2016)

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Jiang et al., 2016)
FBrf0230867-2.coIP.WB
Reference
Jiang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait
Fmr1 

dFMRP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Jiang et al., 2016)
FBrf0230867-3.coIP.WB
Reference
Jiang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMRP

bait

FLAG tag

Fmr1 
Fmr1 
piwi 

Piwi

prey

Myc tag

piwi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fmr1 
N-terminal region
sufficient binding region
aa 1-181
Fmr1 
N-terminal region
necessary binding region
aa 1-181
piwi 
N-terminal region
sufficient binding region
aa 1-510
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2016)
FBrf0230867-4.coIP.WB
Reference
Jiang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait

Myc tag

piwi 
Fmr1 

dFMRP

prey

FLAG tag

Fmr1 
Fmr1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fmr1 
N-terminal region
sufficient binding region
aa 1-181

"N1" construct

Fmr1 
N-terminal region
necessary binding region
aa 1-181

"C1" construct

piwi 
N-terminal region
sufficient binding region
aa 1-510

"N" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jiang et al., 2016)
FBrf0230867-5.PD.WB
Reference
Jiang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMRP

bait

GST tag

Fmr1 
piwi 

Piwi

prey
piwi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fmr1 
N-terminal region
sufficient binding region
aa 1-330

"N2" construct

piwi 
N-terminal region
sufficient binding region
aa 1-510

"N" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Jiang et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)