FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Eps-15, numb
FlyBase ID
FBig0000104116
Interaction Network
Interactions Browser links
Eps-15 network
numb network
Reported Interactions
FBrf0184155-1.PD.WB
Reference
Tang et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Eps-15 

EH1

bait

GST tag

Eps-15 
numb 

Numb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Eps-15 
EH domains
sufficient binding region
aa 1-320
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Tang et al., 2005)
FBrf0184155-3.Y2H
Reference
Tang et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

fused to GAL4 DNA-binding domain

numb 
Eps-15 

EH1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
C-terminus
necessary binding region
aa 336-556
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Tang et al., 2005)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Tang et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)