FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Nbr, piwi
FlyBase ID
FBig0000104118
Interaction Network
Interactions Browser links
Nbr network
piwi network
Reported Interactions
FBrf0230844-1.coIP.MS
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nbr 

Nbr

bait

Myc tag

piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Wang et al., 2016)
FBrf0230844-2.coIP.WB
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nbr 

Nbr

bait

Myc tag

piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Wang et al., 2016)
FBrf0230844-3.coIP.WB
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait
Nbr 

Nbr

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Wang et al., 2016)
FBrf0230844-4.coIP.WB
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Nbr 

Nbr

bait

FLAG tag

piwi 

Piwi

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Nbr coimmunoprecipitates piwi but not aub or AGO3 in this co-transfection and coimmunoprecipitation assay.

(Wang et al., 2016)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2016)
FBrf0234262-1.coIP.WB
Reference
Hayashi et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nbr 

nibbler

bait

FLAG tag

piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Hayashi et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)