FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pc, nej
FlyBase ID
FBig0000104158
Interaction Network
Interactions Browser links
Pc network
nej network
Reported Interactions
FBrf0230961-10.PD.WB
Reference
Tie et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

bait

GST tag

nej 
nej 
nej 
Pc 

PC

prey

His tag

Pc 
Pc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
HAT domain N-terminal
sufficient binding region
aa 1941-2138

weak binding, construct "HAT-N", coordinates relative to nej-PB

nej 
HAT domain central
sufficient binding region
aa 2132-2193

construct "HAT-C2", coordinates relative to nej-PB

nej 
autoinhibitory loop
sufficient binding region
aa 2175-2247

construct "HAT-C2", coordinates relative to nej-PB

Pc 
central region
sufficient binding region
aa 75-228
Pc 
C-terminal region
sufficient binding region
aa 222-390

weak binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Tie et al., 2016)

Several independent interaction regions were identified in both nej and Pc. Pc aa 75-228 binds to the nej AIL domain (aa 2175-2247), while Pc aa 222-390 binds to the region preceeding the nej AIL domain (aa 2132-2193).

(Tie et al., 2016)

Binding of Pc to GST-nej is inhibited by the presence of histones.

(Tie et al., 2016)

In vitro acetylation of nej reduces binding to Pc aa 75-228 fragment.

(Tie et al., 2016)
FBrf0230961-11.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

enzyme

GST tag

nej 

CBP

enzyme

GST tag

Pc 

Pc

inhibitor

His tag

Pc 

Pc

inhibitor

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0230961-3.coIP.WB
Reference
Tie et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pc 

PC

bait
nej 

CBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

nej protein immunoprecipitated with Pc is not acetylated, while all acetylated nej protein is found in the unbound fraction.

(Tie et al., 2016)

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tie et al., 2016)
FBrf0230961-4.coIP.WB
Reference
Tie et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

bait
Pc 

PC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tie et al., 2016)
FBrf0230961-8.PD.WB
Reference
Tie et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

bait

GST tag

nej 
Pc 

PC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
HAT domain
sufficient binding region
aa 1941-2330

coordinates relative to nej-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic nuclear extract.

(Tie et al., 2016)

nej pulls down Pc but not Psc or Su(z)12 in this assay.

(Tie et al., 2016)
FBrf0230961-9.coIP.WB
Reference
Tie et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Pc 

PC

bait
nej 

CBP

prey

FLAG tag

nej 
nej 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
C-terminal region
sufficient binding region
aa 1467-3276

coordinates relative to nej-PB

nej 
HAT domain
necessary binding region
aa 1565-2143

coordinates relative to nej-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tie et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)