FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
brat, dpn
FlyBase ID
FBig0000104159
Interaction Network
Interactions Browser links
brat network
dpn network
Reported Interactions
FBrf0230116-4.coIP.PCR
Reference
Loedige et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

Brat

bait

FLAG tag

dpn 

dpn

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Putative brat-bound transcripts were identified by RIP-chip, then validated by RIP and qRT-PCR.

(Loedige et al., 2015)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Loedige et al., 2015)
FBrf0237716-3.EMSA.A
Reference
Reichardt et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

Brat

bait

His tag

brat 
dpn 

dpn

prey

[32]P label

dpn 
dpn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brat 
NHL domain
sufficient binding region
aa 756-1037
dpn 
3-prime UTR
sufficient binding region
dpn 
3-prime UTR
mutation disrupting interaction

several putative brat binding sites, (A/U)UGUU(A/G/U), in dpn 3-prime UTR, mutations in which abolish interaction.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Reichardt et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)